Nonplasminogen-dependent protease in human plasma
نویسندگان
چکیده
منابع مشابه
Nonplasminogen-dependent protease in human plasma.
Equal volumes of plasma and 0.3 M K2HPO4, pH 7.4, were mixed, diluted 20-fold, and adjusted to pH 5.2. After incubation at 37 degrees C for 30 min, the euglobulin percipitate, redissolved in 0.1 M K2HPO4, pH 7.4, developed caseinolytic activity (0.05 CTA U/ml). Na2HPO4 or NaCl of similar ionic strength could replace K2HPO4. The pH optimum of the protease was 6.5, activity falling off sharply be...
متن کاملNonpiasminogen - Dependent Protease in Human Plasma
Equal volumes of plasma and 0.3 M K2HPO4, pH 7.4, were mixed, diluted 20-fold, and adjusted to pH 5.2. After incubation at 37#{176}C for 30 mm, the euglobulin precipitate, redissolved in 0.1 M K2HPO4, pH 7.4. developed caseinolytic activity (0.05 CTA U/mI). Na2HPO4 or NaCI of similar ionic strength could replace K2HPO4. The pH optimum of the protease was 6.5. activity falling off sharply below ...
متن کاملIsolation of a protein Z-dependent plasma protease inhibitor.
Human protein Z (PZ) is a 62,000-Mr, vitamin K-dependent plasma protein whose structure is similar to coagulation factors VII, IX, X, protein C, and protein S, but whose function is not known. The procoagulant activity of factor Xa in a one-stage plasma coagulation assay is reduced when factor Xa is first incubated with PZ. This apparent inhibitory effect is time dependent, requires the presenc...
متن کاملHuman airway trypsin-like protease stimulates human bronchial fibroblast proliferation in a protease-activated receptor-2-dependent pathway.
Human airway trypsin-like protease (HAT) was isolated from airway secretions and localized to bronchial epithelial cells by immunohistochemistry. In the present study, we examined whether HAT could stimulate DNA synthesis and proliferation of primary human bronchial fibroblasts (HBF). HAT significantly stimulated the proliferation of HBF by 20-55%, a level similar to that of the mitogenic activ...
متن کامل-dependent Protease, Calpain
The activity of integrins on leukocytes is kept under tight control to avoid inappropriate adhesion while these cells are circulating in blood or migrating through tissues. Using lymphocyte function-associated antigen-1 (LFA-1) on T cells as a model, we have investigated adhesion to ligand intercellular adhesion molecule-1 induced by the Ca 2 1 mobilizers, ionomycin, 2,5di-t-butylhydroquinone, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v54.3.607.bloodjournal543607